J996
996 .
CAR BACILLUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
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| 1 Use the Vero E6 cell culture supernatant fluid (see below). Incubate the microorganism in the supernatant fluid in a polystyrene culture flask (75 cm2 flask, canted neck, vent cap, coated with Ultra-Low Attachment Surface, Corning: Cat. No. 3814) at 37C under 5% CO2. |
Equipment needed: Centrifuge
| 1 Incubate Vero E6 cells (ATCC CRL-1586) in Iscove's Modified Dulbecco's Medium (IMDM, GlutaMAXTM) (Life Technologies: Cat. No. 31980030) supplemented with 10% fetal bovine serum (FBS; inactivated at 56C for 30 min) at 37C under 5% CO2. After cultivation, centrifuge the culture medium. Use the supernatant fluid for cultivation of the microorganism as described above. |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Centrifuge |
| Edit in Medium builder | |
Gas composition
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
