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JCM 998 . DESULFOSPOROSINUS ACIDODURANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J998 #

ml
Equipment needed: Anaerobic workbench Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 1.42 g 1.376 9.687
Na2HPO4 x 2 H2O 0.53 g 0.514 3.618
KH2PO4 0.41 g 0.397 2.919
NH4Cl 0.30 g 0.291 5.435
CaCl2 x 2 H2O 0.11 g 0.107 0.725
MgCl2 x 6 H2O 0.10 g 0.097 0.477
NaCl 0.30 g 0.291 4.974
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract
(BD-Difco) 		0.20 g 0.194 -
Resazurin 0.50 mg 4.8e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components thoroughly, bring to a boil for a few seconds and cool down under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
glycerin
(w/v, 5%) 		10.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
2 Comment: For JCM 32882 and JCM 32883, add per liter 10.0 g iron powder in the medium. Then, adjust final pH of the medium to 2.5.

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 2.5
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 1.376 9.687
Na2HPO4 x 2 H2O 0.514 3.618
KH2PO4 0.397 2.919
NH4Cl 0.291 5.435
NaCl 0.291 4.974
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain