Strain modifications for Medium 104b
16 distinct modifications were found.
Adjust pH of complete medium to 7.5.
Adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
Adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
Omit glucose.
Plus 30 mM Lysine, and vitamin mixture as indicated in medium 141; anaerobic
Reduce amount of D-glucose to 2.0 g/l.
Replace glucose by fructose (5g/L); strictly anaerobic
Replace glucose with 1.0 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH to 6.5.
Replace glucose with 2.0 g/l Na-pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration.
Replace glucose with 5.0 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH to 7.4 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere
Replace glucose with 5.0 g/l D-fructose added after autoclaving from an anoxic stock solution and adjust pH to 7.5 - 8.0.
Replace glucose with 5.0 g/l D-fructose added after autoclaving from an anoxic stock solution.
Replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1.0 ml/l of a sterile anoxic seven vitamins solution (see medium 503).
Replace glucose with 5.0 g/l xylan.
Replace glucose with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration.