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DSMZ

Strain modifications for Medium 1069

7 distinct modifications were found.

For DSM 18064. Adjust pH of final medium to 6.8. Add the following components after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised) and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

6.8
3 g/l Sodium pyruvate
1 ml/l Vitamin B12 (2 mg/l filter sterilised)

For DSM 18713 and 18859. Adjust pH of final medium to 7.2. Add the following components after autoclaving from sterile stock solution. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, 6 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

1 ml/l Vitamin B12 (2 mg/l filter sterilised)
1 mM Na2S x 9 H2O
10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2)
6 mM Na2S2O3 x 5 H2O
3 g/l Sodium pyruvate
7.2

For DSM 18985. Adjust amount of MgSO4 x 7 H2O to 1.5 g/l. Adjust pH of final medium to 6.8. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

MgSO4 x 7 H2O
1.5 g/l MgSO4 x 7 H2O
6.8
3 g/l Sodium pyruvate
1 ml/l Vitamin B12 (2 mg/l filter sterilised)
10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2)
1 mM Na2S x 9 H2O (or Na2S2O3 x 5 H2O 2.0 mM)

For DSM 19784. Adjust pH of final medium to 6.9. Add the following components after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), 6 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

3 g/l Sodium pyruvate
1 ml/l Vitamin B12 (2 mg/l filter sterilised)
1 mM Na2S x 9 H2O
6 mM Na2S2O3 x 5 H2O
10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2)
6.9

For DSM 19947. Adjust pH of final medium to 6.9. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, 4 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

6.9
3 g/l Sodium pyruvate
1 ml/l Vitamin B12 (2 mg/l filter sterilised)
1 mM Na2S x 9 H2O
4 mM Na2S2O3 x 5 H2O
10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2)

For DSM 21808 and 21809. Adjust pH of final medium to 7.5. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 3 ml/l Na2S x 9 H2O (1M), 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), and 3 g/l Sodium pyruvate.

Modified strains

Marichromatium sp. DSM 21808

Annotated modifications

7.5
3 g/l Sodium pyruvate
1 ml/l Vitamin B12 (2 mg/l filter sterilised)
3 ml/l Na2S x 9 H2O (1M)
10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2)

For DSM 24766. Adjust amount of MgSO4 x 7 H2O to 1 g/l. Adjust pH of final medium to 7.0. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, and 3 g/l Sodium pyruvate.

Modified strains

Annotated modifications

MgSO4 x 7 H2O
1 g/l MgSO4 x 7 H2O
7.0
3 g/l Sodium pyruvate
1 ml/l Vitamin B12
1 mM Na2S x 9 H2O
10 ml/l NaHCO3
Modifications
For DSM 18064. Adjust pH of final medium to 6.8. Add the following components after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised) and 3 g/l Sodium pyruvate. For DSM 18713 and 18859. Adjust pH of final medium to 7.2. Add the following components after autoclaving from sterile stock solution. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, 6 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate. For DSM 18985. Adjust amount of MgSO4 x 7 H2O to 1.5 g/l. Adjust pH of final medium to 6.8. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, and 3 g/l Sodium pyruvate. For DSM 19784. Adjust pH of final medium to 6.9. Add the following components after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), 6 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate. For DSM 19947. Adjust pH of final medium to 6.9. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, 4 mM Na2S2O3 x 5 H2O, and 3 g/l Sodium pyruvate. For DSM 21808 and 21809. Adjust pH of final medium to 7.5. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 3 ml/l Na2S x 9 H2O (1M), 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), and 3 g/l Sodium pyruvate. For DSM 24766. Adjust amount of MgSO4 x 7 H2O to 1 g/l. Adjust pH of final medium to 7.0. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12, 1 mM Na2S x 9 H2O, 10 ml/l NaHCO3, and 3 g/l Sodium pyruvate.