Strain modifications for Medium 119
12 distinct modifications were found.
Adjust pH of complete medium to 8.5 - 9.0.
Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation.
Adjust pH of complete medium to 7.6.
Adjust pH of complete medium to 8.0 - 8.3.
Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation.
Increase amount of Na-acetate to 3.00 g/l.
Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141).
Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.
Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2.