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DSMZ

Strain modifications for Medium 119

15 distinct modifications were found.

Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0.

Modified strains

Annotated modifications

Na2S x 9 H2O
0.3 g DL-Dithiothreitol
6.0

Adjust pH of final medium to 6.0.

Modified strains

Aceticella autotrophica DSM 108286

Annotated modifications

6.0

Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O.

Modified strains

Methanobacterium congolense DSM 7095

Annotated modifications

0.3 g DL-Dithiothreitol
Na2S x 9 H2O

Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation.

Modified strains

Methanobacterium bryantii DSM 9575

Adjust pH of complete medium to 7.6.

Modified strains

Methanobacterium formicicum DSM 1535

Annotated modifications

7.6

Adjust pH of complete medium to 8.0 - 8.3.

Modified strains

Methanothermobacter sp. DSM 3267
Methanobacterium subterraneum DSM 11074
Methanobacterium subterraneum DSM 11075

Annotated modifications

8.0 - 8.3

Adjust pH of complete medium to 8.5 - 9.0.

Modified strains

Methanobacterium alkalithermotolerans DSM 102889

Annotated modifications

8.5 - 9.0

Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.

Modified strains

Methanobacterium flexile DSM 25939

Annotated modifications

7.2

Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.

Modified strains

Methanobacterium movens DSM 25945

Annotated modifications

7.4

Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation.

Modified strains

Thermoanaerobacter kivui DSM 2030

Annotated modifications

6.5

Increase amount of Na-acetate to 3.00 g/l.

Modified strains

Methanoculleus bourgensis DSM 6216

Annotated modifications

Na-acetate
3.00 g Na-acetate

Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141).

Modified strains

Methanobrevibacter olleyae DSM 16632

Annotated modifications

+ 50.0 ml Clarified rumen fluid
- Sludge fluid
+ 10.0 ml Wolin's vitamin solution
2.00 g Trypticase peptone

Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation.

Modified strains

Methanobrevibacter boviskoreani DSM 25824

Annotated modifications

0.10 g 2-Mercaptoethanesulfonic acid

Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O.

Modified strains

Methanobrevibacter ruminantium DSM 1093
Methanobrevibacter arboriphilus DSM 1125
Methanobrevibacter thaueri DSM 11995
Methanobrevibacter millerae DSM 16643

Annotated modifications

0.30 g DL-Dithiothreitol
+ 1 ml Wolin's vitamin solution (10x)
Na2S x 9 H2O
L-Cysteine HCl x H2O
0.15 g 2-Mercaptoethanesulfonic acid

Supplement medium with 2.00 g/l Na2SO4.

Modified strains

Desulfovibrio sp. DSM 7057

Annotated modifications

2.00 g Na2SO4
Modifications
Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Adjust pH of final medium to 6.0. Adjust pH of final medium to 6.0. Supplement medium with 0.3 g DL-Dithiothreitol. Omit Na2S x 9 H2O. Add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. Adjust pH of complete medium to 7.6. Adjust pH of complete medium to 8.0 - 8.3. Adjust pH of complete medium to 8.5 - 9.0. Adjust pH of the complete medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. Adjust pH of the complete medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. Adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. Increase amount of Na-acetate to 3.00 g/l. Replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310) and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of Wolin's vitamin solution (see medium 141). Supplement medium after autoclaving with 0.10 g/l 2-mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O. Supplement medium with 2.00 g/l Na2SO4.