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DSMZ

Strain modifications for Medium 120b

2 distinct modifications were found.

Supplement medium after autoclaving with 0.5 g/l DTT (DL-dithiothreitol) added from an anoxic stock solution sterilized by filtration. Omit Na2S x 9 H2O and L-cysteine HCl x H2O.

Modified strains

Methanimicrococcus hacksteinii DSM 115570

Annotated modifications

L-Cysteine HCl x H2O
Na2S x 9 H2O
0.5 g DL-Dithiothreitol

Supplement medium with 6.00 g/l NaCl. Do not add overpressure of 80% H2 and 20% CO2 gas mixture.

Modified strains

Methanosarcina subterranea DSM 22503

Annotated modifications

6.00 g NaCl
Modifications
Supplement medium after autoclaving with 0.5 g/l DTT (DL-dithiothreitol) added from an anoxic stock solution sterilized by filtration. Omit Na2S x 9 H2O and L-cysteine HCl x H2O. Supplement medium with 6.00 g/l NaCl. Do not add overpressure of 80% H2 and 20% CO2 gas mixture.