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DSMZ

Strain modifications for Medium 1210a

4 distinct modifications were found.

Omit acetate and supplement medium after autoclaving with 1.00 g/l KNO3 added from a sterile anoxic stock solution prepared under 100% N2 gas.

Modified strains

Thermosulfurimonas marina DSM 104922

Annotated modifications

1 g KNO3
Na-acetate

Prepare medium without acetate, thiosulfate and yeast extract. Supplement medium after autoclaving with 2.50 g/l Fe(III)-citrate added from a sterile anoxic stock solution (10% w/v) prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.0.

Modified strains

Deferrisoma palaeochoriense DSM 29363

Annotated modifications

Na-acetate
Yeast extract
Na2S2O3 x 5 H2O
6.0
2.5 g Fe(III) citrate

Supplement medium after autoclaving with 1.00 g/l KNO3 added from a sterile anoxic stock solution prepared under 100% N2 gas and 5.00 g/l sulfur (powdered) sterilized separately by steaming for 3 hours on each of 3 successive days.

Modified strains

Inmirania thermothiophila DSM 100275

Annotated modifications

5 g Sulfur powder
1 g KNO3

Supplement medium with 0.50 g/l Na-pyruvate and replace thiosulfate with 0.50 g/l sodium pyrosulfite (Na2S2O5) added after autoclaving from freshly prepared anoxic stock solutions sterilized by filtration. Adjust pH of complete medium to 6.0 - 6.5.

Modified strains

Dissulfurirhabdus thermomarina DSM 100025

Annotated modifications

6.0 - 6.5
0.5 g Na-pyruvate
0.5 g Na2S2O5
Na2S2O3 x 5 H2O
Modifications
Omit acetate and supplement medium after autoclaving with 1.00 g/l KNO3 added from a sterile anoxic stock solution prepared under 100% N2 gas. Prepare medium without acetate, thiosulfate and yeast extract. Supplement medium after autoclaving with 2.50 g/l Fe(III)-citrate added from a sterile anoxic stock solution (10% w/v) prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.0. Supplement medium after autoclaving with 1.00 g/l KNO3 added from a sterile anoxic stock solution prepared under 100% N2 gas and 5.00 g/l sulfur (powdered) sterilized separately by steaming for 3 hours on each of 3 successive days. Supplement medium with 0.50 g/l Na-pyruvate and replace thiosulfate with 0.50 g/l sodium pyrosulfite (Na2S2O5) added after autoclaving from freshly prepared anoxic stock solutions sterilized by filtration. Adjust pH of complete medium to 6.0 - 6.5.