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DSMZ

Strain modifications for Medium 194

16 distinct modifications were found.

Na-propionate is replaced by 0.40 g/l 3,4,5-trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution (2% w/v) prepared under N2 and neutralized with NaOH. Stock solutions of 3,4,5-trimethoxybenzoate should be prepared freshly and sterilized by filtration. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration.

Modified strains

Desulfofundulus sp. DSM 7474
Desulfofundulus sp. DSM 7475
Desulfofundulus sp. DSM 7476

Annotated modifications

Na-propionate
+ 1.0 ml Seven vitamins solution
- Solution C
+ 20 ml Solution C
0.40 g 3,4,5-Trimethoxybenzoic acid
to Solution C
Distilled water
to Solution C
20 ml Distilled water
to Solution C

Na-propionate is replaced with 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol.

Modified strains

Desulfovirga adipica DSM 12016

Annotated modifications

Na-propionate
1.00 g Yeast extract
to Solution A
0.10 ml 1-Propanol
to Solution C

Na-propionate is replaced with 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2.

Modified strains

Desulfoprunum benzoelyticum DSM 28570

Annotated modifications

Na-propionate
0.15 g Na-benzoate
to Solution C

Na-propionate is replaced with 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration.

Modified strains

Desulfotomaculum nigrificans DSM 14880

Annotated modifications

Na-propionate
0.5 g/l Yeast extract
to Solution A
2.20 g Na-pyruvate
to Solution C

Na-propionate is replaced with 0.70 g/l ethanol absolute added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, the medium is supplemented with 0.10 g/l each of yeast extract and casamino acids.

Modified strains

Solidesulfovibrio carbinolicus DSM 3852

Annotated modifications

Na-propionate
0.10 g Casamino acids
to Solution A
0.10 g Yeast extract
to Solution A
0.70 g Ethanol absolute
to Solution C

Na-propionate is replaced with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7.

Modified strains

Desulfobotulus sapovorans DSM 2055

Annotated modifications

7.7
Na-propionate
0.15 g Sodium octanoate
to Solution C
0.30 g Na-caproate
to Solution C
0.70 g Na-butyrate
to Solution C

Na-propionate is replaced with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 9.0.

Modified strains

Desulfobotulus mexicanus DSM 105758

Annotated modifications

9.0
Na-propionate
0.15 g Sodium octanoate
to Solution C
0.30 g Na-caproate
to Solution C
0.70 g Na-butyrate
to Solution C

Na-propionate is replaced with 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2.

Modified strains

Anaerovorax odorimutans DSM 5092

Annotated modifications

Na-propionate
0.90 g Putrescine
to Solution C

Na-propionate is replaced with 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2.

Modified strains

Desulforegula conservatrix DSM 13527
Fundidesulfovibrio butyratiphilus DSM 21556
unclassified bacterium DSM 24590

Annotated modifications

Na-propionate
1.00 g Na-butyrate
to Solution C

Na-propionate is replaced with 1.50 g/ 1,2-propanediol added after autoclaving from a sterile, anoxic stock solution prepared under N2.

Modified strains

Desulfovibrio sp. DSM 107641

Annotated modifications

Na-propionate
1.50 g 1,2-propanediol
to Solution C

Na-propionate is replaced with 1.50 g/ Na-benzoate and 1.00 g/l Na-acetate as substrates. Reduce amount of sulfide to 0.10 g/l. Dithionite is used to reduce medium completely.

Modified strains

Syntrophus buswellii DSM 102358
Syntrophus buswellii DSM 102359
Syntrophus buswellii DSM 102360

Annotated modifications

20.00 mg Sodium dithionite
Na-propionate
Na2S x 9 H2O
1.00 g Na-acetate
to Solution C
1.50 g Na-benzoate
to Solution C
0.10 g Na2S x 9 H2O
to Solution E

Na-propionate is replaced with 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2.

Modified strains

Solidesulfovibrio alcoholivorans DSM 5433

Annotated modifications

Na-propionate
1.50 g 1,2-propanediol
to Solution C

Na-propionate is replaced with 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate.

Modified strains

Desulfococcus biacutus DSM 5651

Annotated modifications

Na-propionate
1.50 g Na-3-hydroxybutyrate
to Solution C

Na-propionate is replaced with 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0.

Modified strains

Anaeroarcus burkinensis DSM 6283
Desulfosporomusa polytropa DSM 12907

Annotated modifications

6.8 - 7.0
Na-propionate
1.00 g Yeast extract
to Solution A
2.50 g Na-(DL)-lactate
to Solution C

Na-propionate is replaced with 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration.

Modified strains

Desulfovibrio sp. DSM 24454
Desulfovibrio sp. DSM 10291

Annotated modifications

Na-propionate
+ 1.0 ml Seven vitamins solution
2.50 g Na-L-lactate
to Solution C

Supplement medium with 0.50 g/l yeast extract and replace Na-propionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2.

Modified strains

Salileptolyngbya sp. DSM 5193

Annotated modifications

Na-propionate
0.50 g Yeast extract
to Solution A
0.90 g Acetic acid, 2-methoxy-, sodium salt (1:1)
to Solution C
Modifications
Na-propionate is replaced by 0.40 g/l 3,4,5-trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution (2% w/v) prepared under N2 and neutralized with NaOH. Stock solutions of 3,4,5-trimethoxybenzoate should be prepared freshly and sterilized by filtration. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. Na-propionate is replaced with 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. Na-propionate is replaced with 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. Na-propionate is replaced with 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. Na-propionate is replaced with 0.70 g/l ethanol absolute added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, the medium is supplemented with 0.10 g/l each of yeast extract and casamino acids. Na-propionate is replaced with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. Na-propionate is replaced with 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 9.0. Na-propionate is replaced with 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. Na-propionate is replaced with 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. Na-propionate is replaced with 1.50 g/ 1,2-propanediol added after autoclaving from a sterile, anoxic stock solution prepared under N2. Na-propionate is replaced with 1.50 g/ Na-benzoate and 1.00 g/l Na-acetate as substrates. Reduce amount of sulfide to 0.10 g/l. Dithionite is used to reduce medium completely. Na-propionate is replaced with 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. Na-propionate is replaced with 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. Na-propionate is replaced with 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. Na-propionate is replaced with 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. Supplement medium with 0.50 g/l yeast extract and replace Na-propionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2.