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DSMZ

Strain modifications for Medium 212

5 distinct modifications were found.

Omit butyrate and add to the complete medium 7.00 g/l sucrose from a sterile anoxic stock solution.

Modified strains

Moorella stamsii DSM 26217

Annotated modifications

7 g Sucrose
to Solution C
Na-butyrate
to Solution C
Distilled water
to Solution C
35 ml Distilled water
to Solution C

Replace butyrate with 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration.

Modified strains

Syntrophobacter wolinii DSM 2805

Annotated modifications

Na-butyrate
1 g D-Glucose
to Solution A
1.5 g Na-propionate
to Solution C

Replace butyrate with 1.50 g/l Na-propionate.

Modified strains

Syntrophobacterium sulfatireducens DSM 16706

Annotated modifications

Na-butyrate
1.5 g Na-propionate
to Solution C

Replace butyrate with 2.00 g/l Na-benzoate.

Modified strains

Syntrophus buswellii DSM 102352

Annotated modifications

Na-butyrate
2 g Na-benzoate
to Solution C

Replace Na-butyrate in solution C with 0.61 g Na-stearate. Adjust the amount of distilled water in solution C to 20 ml. Dissolve stearate in water by heating in a water bath.

Modified strains

Syntrophomonas wolfei subsp. saponavida DSM 4212

Annotated modifications

Na-butyrate
to Solution C
0.61 g Na-stearate
to Solution C
Distilled water
to Solution C
20 ml Distilled water
to Solution C
Modifications
Omit butyrate and add to the complete medium 7.00 g/l sucrose from a sterile anoxic stock solution. Replace butyrate with 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Replace butyrate with 1.50 g/l Na-propionate. Replace butyrate with 2.00 g/l Na-benzoate. Replace Na-butyrate in solution C with 0.61 g Na-stearate. Adjust the amount of distilled water in solution C to 20 ml. Dissolve stearate in water by heating in a water bath.