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DSMZ

Strain modifications for Medium 212a

1 distinct modifications were found.

Replace the sugar mix of solution E with the sugar mix of medium 110a. Omit solution C, solution D, and solution H. After inoculation, add a sterile gas mixture of 80% H2 and 20% CO2 to an overpressure of 0.5 bar.

Modified strains

Treponema ruminis DSM 103462

Annotated modifications

- Solution D
- Solution C
- Solution H
+ 10 ml Solution E
0.5 bar 80% H2, 20% CO2
- Solution E
- Sugar mix
- Solution A
+ 947 ml Solution A
+ 10 ml Sugar mix
to Solution E
Modifications
Replace the sugar mix of solution E with the sugar mix of medium 110a. Omit solution C, solution D, and solution H. After inoculation, add a sterile gas mixture of 80% H2 and 20% CO2 to an overpressure of 0.5 bar.