Strain modifications for Medium 311c

Replace fructose with 1.11 g/l N-acetyl-D-glucosamine as substrate added to the autoclaved medium from a filter-sterilized anoxic stock solution.

Replace fructose with 1.35 g/l betaine as substrate; cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Replace fructose with 2.00 g/l D-glucose added to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Replace fructose with 2.50 g/l Na-(DL)-lactate added to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Replace fructose with 3.60 g/l D-mannitol as substrate added to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Replace fructose with 5.00 g/l D-glucose as substrate added to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 7.8 using a sterile anoxic stock solution of 5% (w/v) Na₂CO₃.

Replace fructose with 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate as substrates added to the autoclaved medium from filter-sterilized anoxic stock solutions.

Supplement medium with 0.30 g DL-Dithiothreitol and 20 ml/l methanol (50% v/v). Omit D-fructose, Na₂S x 9 H₂O, and L-Cysteine HCl x H₂O. After inoculation, add 1 bar overpressure of sterile 80% H₂ and 20% CO₂ gas mixture.