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DSMZ

Strain modifications for Medium 339

30 distinct modifications were found.

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Clostridium butyricum DSM 107392
Clostridium chromiireducens DSM 110167
Senimuribacter intestinalis DSM 106208
Mediterraneibacter agrestimuris DSM 111629

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 5 % horse blood.

Modified strains

Enterococcus faecalis DSM 110077

Add-on rumen fluid (50 ml/L) to stimulate growth; anaerobic,plus H2 (0,5 bar)

Modified strains

Bullifex porci DSM 105750

Annotated modifications

50 ml/l Rumen fluid

Anaerobic (N2CO2 plus 0,5 bar H2)

Modified strains

Desulfovibrio porci DSM 105247

Anaerobic (N2CO2 plus 1 bar H2)

Modified strains

Parafannyhessea umbonata DSM 105184

Anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Pyramidobacter porci DSM 105193
Holdemanella porci DSM 105256

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Faecalibaculum rodentium DSM 110083

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Schaalia hyovaginalis DSM 106277
Oliverpabstia intestinalis DSM 106162
Clostridium perfringens DSM 106278
Staphylococcus epidermidis DSM 106280
Peptostreptococcus porci DSM 106284
Megasphaera elsdenii DSM 106891
Selenomonas montiformis DSM 106892
Pseudoramibacter porci DSM 106894
Lactobacillus johnsonii DSM 106897
Parabacteroides distasonis DSM 106899
Bifidobacterium longum subsp. infantis DSM 107246
Clostridium cochlearium DSM 107247
Paraclostridium sp. DSM 107287
Phocaeicola vulgatus DSM 107446
Clostridium sp. DSM 107452
Segatella copri DSM 108494
Hallella mizrahii DSM 108495
Weizmannia agrestimuris DSM 106041
Enterococcus faecalis DSM 106192
Hungatella effluvii DSM 107394
Parasutterella muris DSM 110635

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 5% horse blood.

Modified strains

Lactococcus petauri DSM 109777

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 7 % sheep blood, in co-culture with DSM 105185 Tissierella pigra

Modified strains

Sodaliphilus pleomorphus DSM 108610

Annotated modifications

70.0 g/l Sheep blood
Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood.

Modified strains

Caniella muris DSM 110323

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media add 5% horse blood.

Modified strains

Lactobacillus agrestimuris DSM 110155
Adlercreutzia aquisgranensis DSM 108611
Caniella muris DSM 110983

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media add 7 % horse blood.

Modified strains

Veillonella agrestimuris DSM 110088

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid medium add 5 % horse blood.

Modified strains

Limosilactobacillus antri DSM 110087
Parasutterella sp. DSM 111000

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid medium add 5% horse blood.

Modified strains

Neobacillus muris DSM 110989
Limosilactobacillus caecicola DSM 110982

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). In solid media add 5% horse blood.

Modified strains

Yersinia enterocolitica DSM 110076

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic

Modified strains

Escherichia coli DSM 106279
Fusobacterium mortiferum DSM 108838
Baileyella intestinalis DSM 106896

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Waltera intestinalis DSM 108985

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cysteine
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Tissierella pigra DSM 105185

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; N2

Modified strains

Limosilactobacillus mucosae DSM 102820

Annotated modifications

Sodium resazurin
2 ml/l Phenosafranine
0.05 g/ml Cystein
0.02 g/ml DTT

Plus 2% FBS; anaerobic

Modified strains

Faecalibacterium gallinarum DSM 23680

Annotated modifications

20.0 g/l FBS

Plus NBB concentrate

Modified strains

Olsenella porci DSM 105246

Prereduced; anaerobic

Modified strains

Adlercreutzia equolifaciens subsp. equolifaciens DSM 19450

Suplemented with 0,1 %NaHCO3 (10%); anaerobic

Modified strains

Duncaniella muris DSM 103720

Annotated modifications

0.1 % NaHCO3

Supplement medium after autoclaving with 5.0 g/l D-glucose and 2.0 g/l NaHCO3 added from sterile stock solutions prepared under 100% N2 or 80% N2 and 20% CO2 gas atmosphere, respectively. Adjust pH to 7.0 - 7.2, if necessary.

Modified strains

Clostridium frigoris DSM 14204

Annotated modifications

7.0 - 7.2
5 g D-Glucose
2 g NaHCO3

With 1 g/l of NaHCO3; anaerobic

Modified strains

Anaerobiospirillum succiniciproducens DSM 6400

Annotated modifications

1 g/l NaHCO3

With 1% arginine; anaerobic

Modified strains

Slackia isoflavoniconvertens DSM 22006

Annotated modifications

10.0 g/l Arginine

Adjust pH of complete medium to 7.4.

Modified strains

Anaerocolumna cellulosilytica DSM 100423

Annotated modifications

7.4

Supplement medium after autoclaving with 10.0 g/l D-glucose added from a sterile stock solution.

Modified strains

Clostridium scindens DSM 5676

Annotated modifications

10 g D-Glucose

Use medium at half-strength (16.5 g/l).

Modified strains

Caloramator coolhaasii DSM 12679
Soehngenia saccharolytica DSM 12858
Modifications
Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 5 % horse blood. Add-on rumen fluid (50 ml/L) to stimulate growth; anaerobic,plus H2 (0,5 bar) Anaerobic (N2CO2 plus 0,5 bar H2) Anaerobic (N2CO2 plus 1 bar H2) Anaerobic, N2CO2 plus H2 (1 bar) Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 5% horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Add 7 % sheep blood, in co-culture with DSM 105185 Tissierella pigra Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media 5% horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media add 5% horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media add 7 % horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid medium add 5 % horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid medium add 5% horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). In solid media add 5% horse blood. Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic, N2CO2 plus H2 (1 bar) Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; anaerobic, N2CO2 plus H2 (1 bar) Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).; N2 Plus 2% FBS; anaerobic Plus NBB concentrate Prereduced; anaerobic Suplemented with 0,1 %NaHCO3 (10%); anaerobic Supplement medium after autoclaving with 5.0 g/l D-glucose and 2.0 g/l NaHCO3 added from sterile stock solutions prepared under 100% N2 or 80% N2 and 20% CO2 gas atmosphere, respectively. Adjust pH to 7.0 - 7.2, if necessary. With 1 g/l of NaHCO3; anaerobic With 1% arginine; anaerobic Adjust pH of complete medium to 7.4. Supplement medium after autoclaving with 10.0 g/l D-glucose added from a sterile stock solution. Use medium at half-strength (16.5 g/l).