Strain modifications for Medium 381

29 distinct modifications were found.

Adjust pH of final medium to 6.0.

Modified strains

Annotated modifications

6.0

Add 100g NaCl (10%) for 1000 ml medium.

Modified strains

Marinobacter piscensis DSM 26804

Annotated modifications

100.0 g NaCl
NaCl

Add to medium Na-sesquicarbonate solution (1 mL in 10 mL) to achieve pH 9.5 plus CaCl2. Na-sesquicarbonate solution: NaHCO3 4.2 g, Na2CO3 anh. 5.3 g, dist. water 100 mL

Modified strains

Alkalihalobacillus pseudalcaliphilus DSM 29714

Annotated modifications

9.5

Agar plates with 1% hexadecane. Alternatively, place a drop of hexadecane on the lid of the petridish; Use 3 ml medium 1 or medium 381 for rehydration of freeze dried culture.

Modified strains

Alkanindiges illinoisensis DSM 15370

Annotated modifications

10.0 g/l Hexadecane

If used as phage host, growth at 32°C recommended

Modified strains

Acinetobacter baumannii DSM 101993

Medium for chitin decomposition see reference (9297).

Modified strains

Chitinibacter tainanensis DSM 15459

Or any conventional minimal medium supplemented with 0.2% glucose and thiamine

Modified strains

Escherichia coli DSM 7144

Annotated modifications

2.0 g/l Glucose
2 g/l Thiamin

Plus 0,35% Tween 80

Modified strains

Corynebacterium uropygiale DSM 46817

Annotated modifications

0.35 % Tween 80

Plus 10 mg/L MnSO4

Modified strains

Anoxybacillus flavithermus subsp. yunnanensis DSM 23293

Annotated modifications

10 mg/L MnSO4

Plus 100 mM boric acid

Modified strains

Algoriphagus boritolerans DSM 17298

Annotated modifications

100.0 mM Boric acid

Plus 2% NaCl

Modified strains

Demequina globuliformis DSM 27404

Annotated modifications

20.0 g/l NaCl

Plus 25 mg/l Cm

Modified strains

Escherichia coli DSM 6256

Plus 3% NaCl

Modified strains

Pseudodonghicola xiamenensis DSM 18339

Annotated modifications

30.0 g/l NaCl

Plus 3% NaCl (final concentration)

Modified strains

Rhodococcus marinonascens DSM 43752

Annotated modifications

30.0 g/l NaCl
NaCl

Plus ampicillin 100 mg/ml

Modified strains

Escherichia coli DSM 102053

Annotated modifications

100 mg/ml Ampicillin

Plus Kanamycin 50 mg/ml

Modified strains

Escherichia coli DSM 102052

Annotated modifications

50 mg/ml Kanamycin

Plus 0.2% glucose

Modified strains

Escherichia coli DSM 2304

Annotated modifications

2.0 g/l Glucose

plus 20g/l NaCl

Modified strains

Annotated modifications

20 g/l NaCl

plus 3% NaCl (final concentration)

Modified strains

Rhodococcus sp. DSM 107510

Annotated modifications

30 g/l NaCl
NaCl

Plus 50 mg/L Nalidixic acid

Modified strains

Escherichia coli DSM 18455

Annotated modifications

50 mg/l Nalidixic acid

Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Pseudomonas sp. DSM 12800

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Pseudomonas psychrophila DSM 17535

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Delftia sp. DSM 12781

Rehydrate and grow lyophilized cells in liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Chromobacterium vaccinii DSM 25150

Resuspend and grow freeze-dried cells from ampoules in liquid medium first. Subsequently, cultivation may be carried out in liquid medium and on agar plates.

Modified strains

Pusillimonas sp. DSM 18250

Solified with 15 g/l agar

Modified strains

Paracoccus pantotrophus DSM 12449

Annotated modifications

15.0 g/l Agar

With kanamycin for selection

Modified strains

Escherichia coli DSM 7123

With only 5g/L NaCl and supplemented with 50 µg/mL 5-aminolevulinic acid (ALA)

Modified strains

Escherichia coli DSM 22074

Annotated modifications

5 g/L NaCl
50 mg/l 5-Aminolevulinic acid
NaCl

+ 2% NaCl

Modified strains

Myceligenerans xiligouense DSM 15700
Phycicoccus endophyticus DSM 100020

Annotated modifications

20 g/l NaCl
Modifications
Adjust pH of final medium to 6.0. Add 100g NaCl (10%) for 1000 ml medium. Add to medium Na-sesquicarbonate solution (1 mL in 10 mL) to achieve pH 9.5 plus CaCl2. Na-sesquicarbonate solution: NaHCO3 4.2 g, Na2CO3 anh. 5.3 g, dist. water 100 mL Agar plates with 1% hexadecane. Alternatively, place a drop of hexadecane on the lid of the petridish; Use 3 ml medium 1 or medium 381 for rehydration of freeze dried culture. If used as phage host, growth at 32°C recommended Medium for chitin decomposition see reference (9297). Or any conventional minimal medium supplemented with 0.2% glucose and thiamine Plus 0,35% Tween 80 Plus 10 mg/L MnSO4 Plus 100 mM boric acid Plus 2% NaCl Plus 25 mg/l Cm Plus 3% NaCl Plus 3% NaCl (final concentration) Plus ampicillin 100 mg/ml Plus Kanamycin 50 mg/ml Plus 0.2% glucose plus 20g/l NaCl plus 3% NaCl (final concentration) Plus 50 mg/L Nalidixic acid Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells in liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates Resuspend and grow freeze-dried cells from ampoules in liquid medium first. Subsequently, cultivation may be carried out in liquid medium and on agar plates. Solified with 15 g/l agar With kanamycin for selection With only 5g/L NaCl and supplemented with 50 µg/mL 5-aminolevulinic acid (ALA) + 2% NaCl