Strain modifications for Medium 514

119 distinct modifications were found.

(NaCl 50g/l)

Modified strains

Gracilibacillus lacisalsi DSM 19029

Annotated modifications

NaCl
50 g/l NaCl

1/10 concentrated with MnSO4; pH:8.0

Modified strains

Caldalkalibacillus uzonensis DSM 17740

Annotated modifications

8.0

1/10 strength only! plus MnSO4; pH 8.0

Modified strains

Thermaerobacter subterraneus DSM 13965

Annotated modifications

8.0

10fold diluted

Modified strains

: use diluted medium: 40 ml medium 514 plus 60 ml artificial sea water

Modified strains

Planktomarina temperata DSM 22400

Add 1% sodium pyruvate

Modified strains

Marinobacter sp. DSM 26291
Alcanivorax sp. DSM 26292
Alcanivorax sp. DSM 26293
Alcanivorax sp. DSM 26294
Alcanivorax sp. DSM 26295
Pseudoalteromonas sp. DSM 26664
Pseudoalteromonas sp. DSM 26666
Halomonas sp. DSM 26667
Cycloclasticus sp. DSM 27168

Annotated modifications

10.0 g/l Sodium pyruvate

Add 1% Tween 80

Modified strains

Thalassolituus oleivorans DSM 14913

Annotated modifications

10.0 g/l Tween 80

Add 1g/l sodium acetat

Modified strains

Pseudoalteromonas spiralis DSM 16099

Annotated modifications

1.0 g/l Sodium acetate

Add 3% NaCl

Modified strains

Psychrobacter submarinus DSM 14161

Annotated modifications

30.0 g/l NaCl

Add 4% NaCl

Modified strains

Kushneria indalinina DSM 14324

Annotated modifications

40.0 g/l NaCl

Add 6% NaCl

Modified strains

Marinobacter nauticus DSM 8798

Annotated modifications

60.0 g/l NaCl

Add 8% NaCl

Modified strains

Gramella gaetbulicola DSM 23082

Annotated modifications

80.0 g/l NaCl

Add to the medium after autoclaving 1.00 g/l Na2S2O3 x 5 H2O from a stock solution sterilized by filtration. Check medium pH after autoclaving and adjust if necessary.

Modified strains

Aeropyrum pernix DSM 11879
Aeropyrum camini DSM 16960

Annotated modifications

1.0 g/l Na2S2O3 x 5 H2O

Adjust pH to 8.0

Modified strains

Pseudohaliea rubra DSM 19751

Annotated modifications

8.0

All components (except agar) half strength

Modified strains

Lewinella lutea DSM 102254

Batch quality of the broth and agar is of importance. At DSMZ, media from Bekton Dickinson were suitable

Modified strains

Fulvitalea axinellae DSM 100852

dilute medium 1:10 and add 3 g/l sodium pyruvate

Modified strains

Diluted 1:1

Modified strains

Rheinheimera chironomi DSM 18694

for cultivation in liquid add 50mM galactose

Modified strains

For the liquid medium add 0,2% agar

Modified strains

Catenovulum agarivorans DSM 23111

Annotated modifications

2.0 g/l Agar

Keep agar surface moist

Modified strains

Halioglobus pacificus DSM 27932

Keep agar surface moist or use agar slants submersed under 2 ml marine broth

Modified strains

Lutibacter profundi DSM 100437

Obligately anaerobic

Modified strains

Hoeflea siderophila DSM 21587

Only for solid medium

Modified strains

Boseongicola aestuarii DSM 110602

Only the brand Difco (Difco 2216) supported growth in our lab

Modified strains

Wenyingzhuangia heitensis DSM 101599

PH 6-7

Modified strains

Rheinheimera nanhaiensis DSM 22401

Annotated modifications

6.0 - 7.0

PH 7

Modified strains

Shewanella dokdonensis DSM 23626

Annotated modifications

7.0

PH 7.0

Modified strains

Mycetocola sp. DSM 22605
Piscicoccus intestinalis DSM 22761
Mobilicoccus pelagius DSM 22762
Oceanibacterium hippocampi DSM 23444
Phycicoccus sp. DSM 23465
Microbacterium sp. DSM 23466

Annotated modifications

7.0

Ph 7.0 (Difco 2216)

Modified strains

Thermaerobacter nagasakiensis DSM 14512

Annotated modifications

7.0

PH 7.0-8.0

Modified strains

Bacillus sp. DSM 22499
Pontibacillus sp. DSM 22500
Pontibacillus sp. DSM 22565
Tessaracoccus oleiagri DSM 22955

Annotated modifications

7.0 - 8.0

PH 7.2 - 7.4

Modified strains

Salinicoccus sesuvii DSM 23267

Annotated modifications

7.2 - 7.4

PH 7.5

Modified strains

Shouchella xiaoxiensis DSM 21943
Yaniella soli DSM 22211
Jeotgalicoccus nanhaiensis DSM 23006

Annotated modifications

7.5

PH 7.5 - 7.9

Modified strains

Halomonas sp. DSM 21905

Annotated modifications

7.5 - 7.9

PH 7.6

Modified strains

Robertmurraya beringensis DSM 22571
Salinicoccus kekensis DSM 23173

Annotated modifications

7.6

PH 7.6; 5-7 d

Modified strains

Marisediminicola antarctica DSM 22350

Annotated modifications

7.6

PH 8.5

Modified strains

Nesterenkonia suensis DSM 22748

Annotated modifications

8.5

ph 9.0

Modified strains

PH: 9.0

Modified strains

Nesterenkonia aurantiaca DSM 27373

Annotated modifications

9.0

PH:7-9

Modified strains

Marihabitans asiaticum DSM 18935

Annotated modifications

7.0 - 9.0

Plus 0.1% glucose; facultative anaerobic

Modified strains

Marinilactibacillus piezotolerans DSM 16108

Annotated modifications

1.0 g/l Glucose

Plus 1% Na acetate

Modified strains

Pseudoalteromonas telluritireducens DSM 16098

Annotated modifications

10.0 g/l Na acetate

Plus 1% sodium pyruvate

Modified strains

Oleispira antarctica DSM 14852

Annotated modifications

10.0 g/l Sodium pyruvate

Plus 10 g/l Na-pyruvate

Modified strains

Alcanivorax dieselolei DSM 16502

Annotated modifications

10.0 g/l Na-pyruvate

Plus 10 mg MnSO4/l

Modified strains

Metabacillus indicus DSM 16189

Annotated modifications

10.0 mg MnSO4

Plus 10 mg/l MnSO4

Modified strains

Pontibacillus marinus DSM 16465
Bacillus sp. DSM 17544
Gracilibacillus sp. DSM 22173
Cytobacillus luteolus DSM 22388
Bacillus sp. DSM 17334
Bacillus sp. DSM 17335
Bacillus sp. DSM 17336
Bacillus sp. DSM 17346
Salipaludibacillus neizhouensis DSM 19794
Oceanobacillus kimchii DSM 23341
Virgibacillus byunsanensis DSM 23507
Bacillus siamensis DSM 25261

Annotated modifications

10.0 mg/l MnSO4

Plus 10 mg/l MnSO4; nature

Modified strains

Bacillus sp. DSM 22176

Annotated modifications

10.0 mg/l MnSO4

Plus 10 mg/L MnSO4; pH 7.4

Modified strains

Gracilibacillus marinus DSM 23372

Annotated modifications

10 mg/L MnSO4
7.4

Plus 10 mg/l MnSO4; pH 7.5

Modified strains

Bacillus sp. DSM 22230
Thalassobacillus sp. DSM 22231
Thalassobacillus sp. DSM 22232
Alkalihalobacillus hemicentroti DSM 23007
Shouchella hunanensis DSM 23008
Fictibacillus nanhaiensis DSM 23009
Sutcliffiella zhanjiangensis DSM 23010

Annotated modifications

10.0 mg/l MnSO4
7.5

Plus 10 mg/ml MnSO4

Modified strains

Bacillus sp. DSM 17337

Annotated modifications

10 mg/ml MnSO4

Plus 10 mg/ml MnSO4; pH 7.2

Modified strains

Thalassobacillus pellis DSM 22784

Annotated modifications

10 mg/ml MnSO4
7.2

Plus 10% MRS medium, pH:8.0, aerob-microaerophilic

Modified strains

Marinilactibacillus psychrotolerans DSM 19582

Annotated modifications

100.0 g/l MRS medium
8.0

Plus 10% NaCl

Modified strains

Sediminibacillus albus DSM 19340
Aliifodinibius roseus DSM 21986
Bacillus sp. DSM 24173
Gracilibacillus aidingensis DSM 104330
Aidingibacillus halophilus DSM 104332

Annotated modifications

100.0 g/l NaCl

Plus 100 g/L NaCl

Modified strains

Lentibacillus jeotgali DSM 23924

Annotated modifications

100.0 g/l NaCl

Plus 15 g/L agar

Modified strains

Paenibacillus sp. DSM 23489

Annotated modifications

15 g/L Agar

Plus 2.5% NaCl

Modified strains

Salipiger mucosus DSM 16094
Halomonas anticariensis DSM 16096
Bacillus sp. DSM 28946
Bacillus sp. DSM 28947
Sediminibacillus terrae DSM 28949
Virgibacillus sp. DSM 28951
Bacillus sp. DSM 28962

Annotated modifications

25.0 g/l NaCl

Plus 3% NaCl plus MnSO4

Modified strains

Virgibacillus dokdonensis DSM 16826

Annotated modifications

30.0 g/l NaCl

Plus 30 g/l NaCl

Modified strains

Halomonas ventosae DSM 15911
Halobacillus yeomjeoni DSM 17110
Halomonas kribbensis DSM 17892
Pontibacillus halophilus DSM 19796
Sediminimonas qiaohouensis DSM 21189
Sediminimonas qiaohouensis DSM 21190
Salinarimonas rosea DSM 21201
Actinopolyspora sp. DSM 45297
Halomonas maura DSM 13445
Aidingimonas halophila DSM 19939
Halomonas almeriensis DSM 23775
Metabacillus halosaccharovorans DSM 25387
Gracilibacillus sp. DSM 19226
Filobacillus sp. DSM 19795
Salirhabdus salicampi DSM 19924
Lentibacillus saliphilus DSM 21375

Annotated modifications

30.0 g/l NaCl

Plus 4% NaCl

Modified strains

Oceanobacillus halophilus DSM 23996
Chromohalobacter nigrandesensis DSM 14323
Salinicola halophilus DSM 14325
Marinobacterium halophilum DSM 17586
Pseudoalteromonas marina DSM 17587

Annotated modifications

40.0 g/l NaCl

Plus 4% NaCl; aerobic

Modified strains

Thalassobacillus devorans DSM 16966

Annotated modifications

40.0 g/l NaCl

Plus 40g/L NaCl in addition

Modified strains

Alteribacillus iranensis DSM 23995

Annotated modifications

40.0 g/l NaCl

Plus 5% NaCl

Modified strains

Halobacillus seohaensis DSM 22302
Virgibacillus sediminis DSM 19797
Oceanobacillus locisalsi DSM 27649
Thalassobacillus hwangdonensis DSM 27650
Virgibacillus sp. DSM 19925
Bacillus sp. DSM 21178

Annotated modifications

50.0 g/l NaCl

Plus 5% NaCl and agar (15 g/l)

Modified strains

Georgenia halophila DSM 21365

Annotated modifications

50.0 g/l NaCl
15.0 g/l Agar

Plus 5% NaCl; pH 7.2

Modified strains

Salimicrobium salexigens DSM 22782

Annotated modifications

50.0 g/l NaCl
7.2

Plus 6% NaCl

Modified strains

Halomonas boliviensis DSM 15517
Chromohalobacter sarecensis DSM 15547
Halomonas neptunia DSM 15720
Halomonas meridiana DSM 15721
Halomonas sulfidaeris DSM 15722
Halomonas axialensis DSM 15723
Halomonas meridiana DSM 15724
Halomonas hydrothermalis DSM 15725
Halomonas sp. DSM 9564
Halomonas organivorans DSM 16226
Halomonas alkaliphila DSM 16354
Halomonas kenyensis DSM 17331
Halomonas mongoliensis DSM 17332
Halomonas olivaria DSM 19074
Alkalicoccus urumqiensis DSM 29145
Alkalicoccus halolimnae DSM 29191
Alkalicoccus halolimnae DSM 29242

Annotated modifications

60.0 g/l NaCl

Plus 6% NaCl (w/v), pH 7.5

Modified strains

Halomonas magadiensis DSM 15367

Annotated modifications

60.0 g/l NaCl
7.5

Plus 7% NaCl

Modified strains

Gracilibacillus eburneus DSM 23710

Annotated modifications

70.0 g/l NaCl

Plus 7% NaCl plus 10 mg/l MnSO4; pH 7.2-7.5

Modified strains

Lentibacillus persicus DSM 22530

Annotated modifications

70.0 g/l NaCl
10.0 mg/l MnSO4
7.2 - 7.5

Plus 7% NaCl plus 10 mg/l MnSO4; pH 8.0

Modified strains

Halobacillus sp. DSM 22432

Annotated modifications

70.0 g/l NaCl
10.0 mg/l MnSO4
8.0

Plus 7% NaCl plus 10 mg/ml MnSO4; pH 7.5

Modified strains

Virgibacillus subterraneus DSM 22441

Annotated modifications

70.0 g/l NaCl
10 mg/ml MnSO4
7.5

Plus 8% NaCl

Modified strains

Halomonas alkaliantarctica DSM 15686
Lentibacillus salis DSM 16817
Halomonas sabkhae DSM 19122
Microbulbifer sp. DSM 19217
Halomonas sp. DSM 19218
Aidingimonas halophila DSM 19219
Halomonas sp. DSM 19220
Halomonas lutea DSM 19221
Halomonas korlensis DSM 19633
Halomonas denitrificans DSM 18045
Salimicrobium flavidum DSM 23127
Lentibacillus juripiscarius DSM 16577
Bacillus sp. DSM 16815
Bacillus sp. DSM 16819
Virgibacillus sp. DSM 16820
Gracilibacillus sp. DSM 16821
Bacillus sp. DSM 23712

Annotated modifications

80.0 g/l NaCl

Plus 8% NaCl plus 10 mg/l MnSO4

Modified strains

Lentibacillus salarius DSM 16459

Annotated modifications

10.0 mg/l MnSO4
80.0 g/l NaCl

Plus 8% NaCl plus MnSO4

Modified strains

Alkalibacillus salilacus DSM 16460
Lentibacillus lacisalsi DSM 16462

Annotated modifications

80.0 g/l NaCl

Plus 8% NaCl; aerobic

Modified strains

Halomonas taeanensis DSM 16463

Annotated modifications

80.0 g/l NaCl

Plus 80 g/L NaCL (10% in total); pH 8.0

Modified strains

Oceanobacillus rekensis DSM 26900
Oceanobacillus damuensis DSM 26901

Annotated modifications

80 g/L NaCl
8.0

Plus 80 g/L NaCL; pH 8.0

Modified strains

Alkalibacillus almallahensis DSM 27545

Annotated modifications

80 g/L NaCl
8.0

Plus agar (15 g/l); (slow growing strain, needs 5-8 days on agar plates

Modified strains

Ilumatobacter fluminis DSM 18936

Annotated modifications

15.0 g/l Agar

Plus glucose (10g/L); microaerophilic

Modified strains

Streptohalobacillus salinus DSM 22440

Annotated modifications

10.0 g/l Glucose

Plus MnSO4

Modified strains

Bacillus sp. DSM 14014
Virgibacillus necropolis DSM 14866
Oceanobacillus picturae DSM 14867
Virgibacillus carmonensis DSM 14868
Paraliobacillus ryukyuensis DSM 15140
Aeribacillus pallidus DSM 15447
Pontibacillus chungwhensis DSM 16287
Metabacillus litoralis DSM 16303
Lentibacillus salicampi DSM 16425
Halobacillus locisalis DSM 16468
Bacillus sp. DSM 11708
Bacillus sp. DSM 11709
Ornithinibacillus californiensis DSM 16628
Virgibacillus kekensis DSM 17056
Gracilibacillus quinghaiensis DSM 17858
Virgibacillus olivae DSM 18098
Terribacillus goriensis DSM 18252
Salirhabdus euzebyi DSM 19612
Halobacillus faecis DSM 21559
Terribacillus saccharophilus DSM 21619
Terribacillus halophilus DSM 21620

Plus MnSO4 (10 mg/l)

Modified strains

Bacillus sp. DSM 17333
Bacillus sp. DSM 17548
Bacillus sp. DSM 17549
Bacillus sp. DSM 17550
Paenibacillus sp. DSM 17551

Annotated modifications

10.0 g/l MnSO4

Plus MnSO4 (10mg/l)

Modified strains

Bacillus seohaeanensis DSM 16464
Bacillus sp. DSM 17546
Bacillus sp. DSM 17547

Annotated modifications

10.0 mg/l MnSO4

Plus MnSO4 (see medium 1)

Modified strains

Halobacillus kuroshimensis DSM 18393
Halobacillus profundi DSM 18394

Plus NaCl (100g/l)

Modified strains

Salinicoccus salitudinis DSM 17846

Annotated modifications

100.0 g/l NaCl

Plus NaCl (150 g/L)

Modified strains

Gracilibacillus halophilus DSM 17856

Annotated modifications

150.0 g/l NaCl

Plus NaCl (3%)

Modified strains

Alkalihalophilus lindianensis DSM 26864

Annotated modifications

30.0 g/l NaCl

Plus starch (1%)

Modified strains

Rubrobacter aplysinae DSM 27440

Annotated modifications

10.0 g/l Starch

Plus 0.1% NaCl plus 5% horse or sheep blood

Modified strains

Arcobacter halophilus DSM 18005

Annotated modifications

1.0 g/l NaCl
50.0 g/l Blood

plus 1% glucose

Modified strains

Plus 1% Na pyruvate

Modified strains

Alcanivorax venustensis DSM 13974

Annotated modifications

10.0 g/l Na pyruvate

plus 1% Na-pyruvic acid

Modified strains

plus 10 mg/ml MnSO4

Modified strains

plus 10 µg/ml MnSO4

Modified strains

plus 10% NaCl

Modified strains

plus 10mg/l MnSO4

Modified strains

Plus 4% NaCl

Modified strains

Marinobacter vinifirmus DSM 17747

Annotated modifications

40.0 g/l NaCl

Plus 5% NaCl

Modified strains

Gracilibacillus saliphilus DSM 19802
Pontibacillus sp. DSM 103397
Alteribacillus sp. DSM 103396
Alteribacillus sp. DSM 103398
Bacillus sp. DSM 103400

Annotated modifications

50.0 g/l NaCl

plus 5%-10% NaCl

Modified strains

plus 60 g/L NaCl

Modified strains

Plus 8% NaCl

Modified strains

Halomonas boliviensis DSM 15516
Salibacterium salarium DSM 16461

Annotated modifications

80.0 g/l NaCl

plus MnSO4

Modified strains

prepared in 1/10 strength: 3.74 g MARINE BROTH DIFCO 2216, 15 g agar, 1000 ml 90% artificial seawater

Modified strains

Reactivate freeze dried cells in liquid medium, afterwards, cultivation may be carried out in liquid medium or on agar

Modified strains

Salinivibrio costicola DSM 11403

Rehydrate and grow cells from freeze dried ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid medium or on agar plates.

Modified strains

Vibrio neonatus DSM 17531

Rehydrate and grow freeze dried cells from ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid broth or on agar plates

Modified strains

Enterovibrio calviensis DSM 14347

Rehydrate and grow freeze dried cells in liquid medium 514 first. Subsequent subculturing may be carried out in medium 514 or tryptone soya broth (medium 545) or on agar media.

Modified strains

Marinospirillum sp. DSM 9662

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1303 or 514. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Granulosicoccus antarcticus DSM 24912

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 514. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Photobacterium damselae subsp. piscicida DSM 22834

Supplemented with 1 g/l pancreatic digest of casein, 1 g/l soya peptone plus 1 g/l malt extract

Modified strains

Marinobacter zhanjiangensis DSM 21077
Marinobacter sp. DSM 21168

Annotated modifications

1.0 g/l Malt extract
1.0 g/l Pancreatic digest of casein

Supplemented with glucose (1g/L) and vitamin solution of medium 141 (10ml/L); microaerophilic

Modified strains

Schinkia oryziterrae DSM 26460

Annotated modifications

1.0 g/l Glucose
+ 10.0 ml Wolin's vitamin solution

Takes about 4 weeks to grow

Modified strains

Octadecabacter arcticus DSM 13978
Octadecabacter antarcticus DSM 28635

use carbon sources diluted 1:10

Modified strains

Weak growth

Modified strains

Psychrobacter sanguinis DSM 23635

With 1 % Na acetate

Modified strains

Alcanivorax jadensis DSM 12178

Annotated modifications

10.0 g/l Na acetate

With 1 % sodium pyruvate

Modified strains

Alcanivorax borkumensis DSM 11573

Annotated modifications

10.0 g/l Sodium pyruvate

With 1 g/l yeast extract

Modified strains

Vibrio tapetis DSM 21475

Annotated modifications

1.0 g/l Yeast extract

With 10 mg/L MnSO4

Modified strains

Metabacillus galliciensis DSM 21539

Annotated modifications

10 mg/L MnSO4

With 10% NaCl

Modified strains

Halovivax ruber DSM 18193

Annotated modifications

100.0 g/l NaCl

add with 3 g/l yeast extract, 10 g/l malt extract , 4 g/l Glucose

Modified strains

Nocardioides salarius DSM 22259

Annotated modifications

3.0 g Yeast extract
10.0 g/l Malt extract
4.0 g/l Glucose

mix 700 ml marine broth with 300 ml R2A broth, pH 6.5

Modified strains

Ornithinibacter aureus DSM 23364

PH 10

Modified strains

Lipingzhangella halophila DSM 102030
Natronoglycomyces albus DSM 106290

Annotated modifications

10.0
Modifications
(NaCl 50g/l) 1/10 concentrated with MnSO4; pH:8.0 1/10 strength only! plus MnSO4; pH 8.0 10fold diluted : use diluted medium: 40 ml medium 514 plus 60 ml artificial sea water Add 1% sodium pyruvate Add 1% Tween 80 Add 1g/l sodium acetat Add 3% NaCl Add 4% NaCl Add 6% NaCl Add 8% NaCl Add to the medium after autoclaving 1.00 g/l Na2S2O3 x 5 H2O from a stock solution sterilized by filtration. Check medium pH after autoclaving and adjust if necessary. Adjust pH to 8.0 All components (except agar) half strength Batch quality of the broth and agar is of importance. At DSMZ, media from Bekton Dickinson were suitable dilute medium 1:10 and add 3 g/l sodium pyruvate Diluted 1:1 for cultivation in liquid add 50mM galactose For the liquid medium add 0,2% agar Keep agar surface moist Keep agar surface moist or use agar slants submersed under 2 ml marine broth Obligately anaerobic Only for solid medium Only the brand Difco (Difco 2216) supported growth in our lab PH 6-7 PH 7 PH 7.0 Ph 7.0 (Difco 2216) PH 7.0-8.0 PH 7.2 - 7.4 PH 7.5 PH 7.5 - 7.9 PH 7.6 PH 7.6; 5-7 d PH 8.5 ph 9.0 PH: 9.0 PH:7-9 Plus 0.1% glucose; facultative anaerobic Plus 1% Na acetate Plus 1% sodium pyruvate Plus 10 g/l Na-pyruvate Plus 10 mg MnSO4/l Plus 10 mg/l MnSO4 Plus 10 mg/l MnSO4; nature Plus 10 mg/L MnSO4; pH 7.4 Plus 10 mg/l MnSO4; pH 7.5 Plus 10 mg/ml MnSO4 Plus 10 mg/ml MnSO4; pH 7.2 Plus 10% MRS medium, pH:8.0, aerob-microaerophilic Plus 10% NaCl Plus 100 g/L NaCl Plus 15 g/L agar Plus 2.5% NaCl Plus 3% NaCl plus MnSO4 Plus 30 g/l NaCl Plus 4% NaCl Plus 4% NaCl; aerobic Plus 40g/L NaCl in addition Plus 5% NaCl Plus 5% NaCl and agar (15 g/l) Plus 5% NaCl; pH 7.2 Plus 6% NaCl Plus 6% NaCl (w/v), pH 7.5 Plus 7% NaCl Plus 7% NaCl plus 10 mg/l MnSO4; pH 7.2-7.5 Plus 7% NaCl plus 10 mg/l MnSO4; pH 8.0 Plus 7% NaCl plus 10 mg/ml MnSO4; pH 7.5 Plus 8% NaCl Plus 8% NaCl plus 10 mg/l MnSO4 Plus 8% NaCl plus MnSO4 Plus 8% NaCl; aerobic Plus 80 g/L NaCL (10% in total); pH 8.0 Plus 80 g/L NaCL; pH 8.0 Plus agar (15 g/l); (slow growing strain, needs 5-8 days on agar plates Plus glucose (10g/L); microaerophilic Plus MnSO4 Plus MnSO4 (10 mg/l) Plus MnSO4 (10mg/l) Plus MnSO4 (see medium 1) Plus NaCl (100g/l) Plus NaCl (150 g/L) Plus NaCl (3%) Plus starch (1%) Plus 0.1% NaCl plus 5% horse or sheep blood plus 1% glucose Plus 1% Na pyruvate plus 1% Na-pyruvic acid plus 10 mg/ml MnSO4 plus 10 µg/ml MnSO4 plus 10% NaCl plus 10mg/l MnSO4 Plus 4% NaCl Plus 5% NaCl plus 5%-10% NaCl plus 60 g/L NaCl Plus 8% NaCl plus MnSO4 prepared in 1/10 strength: 3.74 g MARINE BROTH DIFCO 2216, 15 g agar, 1000 ml 90% artificial seawater Reactivate freeze dried cells in liquid medium, afterwards, cultivation may be carried out in liquid medium or on agar Rehydrate and grow cells from freeze dried ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid medium or on agar plates. Rehydrate and grow freeze dried cells from ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid broth or on agar plates Rehydrate and grow freeze dried cells in liquid medium 514 first. Subsequent subculturing may be carried out in medium 514 or tryptone soya broth (medium 545) or on agar media. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1303 or 514. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 514. Subsequent subculturing may be carried out in liquid medium or on agar plates Supplemented with 1 g/l pancreatic digest of casein, 1 g/l soya peptone plus 1 g/l malt extract Supplemented with glucose (1g/L) and vitamin solution of medium 141 (10ml/L); microaerophilic Takes about 4 weeks to grow use carbon sources diluted 1:10 Weak growth With 1 % Na acetate With 1 % sodium pyruvate With 1 g/l yeast extract With 10 mg/L MnSO4 With 10% NaCl add with 3 g/l yeast extract, 10 g/l malt extract , 4 g/l Glucose mix 700 ml marine broth with 300 ml R2A broth, pH 6.5 PH 10