Strain modifications for Medium 514

(NaCl 50g/l)

1/10 concentrated with MnSO₄; pH:8.0

1/10 strength only! plus MnSO₄; pH 8.0

10fold diluted

: use diluted medium: 40 ml medium 514 plus 60 ml artificial sea water

Add 1% sodium pyruvate

Add 1% Tween 80

Add 1g/l sodium acetat

Add 3% NaCl

Add 4% NaCl

Add 6% NaCl

Add 8% NaCl

add one night before use: L-Cystein-HCL at a final concentration of 0.2mg/mL

Add to the medium after autoclaving 1.00 g/l Na₂S₂O₃ x 5 H₂O from a stock solution sterilized by filtration. Check medium pH after autoclaving and adjust if necessary.

Adjust pH to 8.0

All components (except agar) half strength

Batch quality of the broth and agar is of importance. At DSMZ, media from Bekton Dickinson were suitable

dilute medium 1:10 and add 3 g/l sodium pyruvate

Diluted 1:1

for cultivation in liquid add 50mM galactose

For the liquid medium add 0,2% agar

Keep agar surface moist

Keep agar surface moist or use agar slants submersed under 2 ml marine broth

Only for solid medium

Only the brand Difco (Difco 2216) supported growth in our lab

PH 6-7

PH 7

PH 7.0

Ph 7.0 (Difco 2216)

PH 7.0-8.0

PH 7.2 - 7.4

PH 7.5

PH 7.5 - 7.9

PH 7.6

PH 7.6; 5-7 d

PH 8.5

ph 9.0

PH: 9.0

Plus 0.1% glucose; facultative anaerobic

Plus 1% Na acetate

Plus 1% sodium pyruvate

Plus 10 g/l Na-pyruvate

Plus 10 mg MnSO₄/l

Plus 10 mg/l MnSO₄

Plus 10 mg/l MnSO₄; nature

Plus 10 mg/L MnSO₄; pH 7.4

Plus 10 mg/l MnSO₄; pH 7.5

Plus 10 mg/ml MnSO₄

Plus 10 mg/ml MnSO₄; pH 7.2

Plus 10% MRS medium, pH:8.0, aerob-microaerophilic

Plus 10% NaCl

Plus 100 g/L NaCl

Plus 15 g/L agar

Plus 2.5% NaCl

Plus 3% NaCl plus MnSO₄

Plus 30 g/l NaCl

Plus 4% NaCl

Plus 4% NaCl; aerobic

Plus 40g/L NaCl in addition

Plus 5% NaCl

Plus 5% NaCl and agar (15 g/l)

Plus 5% NaCl; pH 7.2

Plus 6% NaCl

Plus 6% NaCl (w/v), pH 7.5

Plus 7% NaCl

Plus 7% NaCl plus 10 mg/l MnSO₄; pH 7.2-7.5

Plus 7% NaCl plus 10 mg/l MnSO₄; pH 8.0

Plus 7% NaCl plus 10 mg/ml MnSO₄; pH 7.5

Plus 8% NaCl

Plus 8% NaCl plus 10 mg/l MnSO₄

Plus 8% NaCl plus MnSO₄

Plus 8% NaCl; aerobic

Plus 80 g/L NaCL (10% in total); pH 8.0

Plus 80 g/L NaCL; pH 8.0

Plus agar (15 g/l); (slow growing strain, needs 5-8 days on agar plates

Plus glucose (10g/L); microaerophilic

Plus MnSO₄

Plus MnSO₄ (10 mg/l)

Plus MnSO₄ (10mg/l)

Plus MnSO₄ (see medium 1)

Plus NaCl (100g/l)

Plus NaCl (150 g/L)

Plus NaCl (3%)

Plus starch (1%)

Plus 0.1% NaCl plus 5% horse or sheep blood

plus 1% glucose

Plus 1% Na pyruvate

plus 1% Na-pyruvic acid

plus 10 mg/ml MnSO₄

plus 10 µg/ml MnSO₄

plus 10% NaCl

plus 10mg/l MnSO₄

Plus 4% NaCl

Plus 5% NaCl

plus 5%-10% NaCl

plus 60 g/L NaCl

Plus 8% NaCl

plus MnSO₄

prepared in 1/10 strength: 3.74 g MARINE BROTH DIFCO 2216, 15 g agar, 1000 ml 90% artificial seawater

Reactivate freeze dried cells in liquid medium, afterwards, cultivation may be carried out in liquid medium or on agar

Rehydrate and grow cells from freeze dried ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid medium or on agar plates.

Rehydrate and grow freeze dried cells from ampoule in 5 ml liquid medium 514. Subsequent subcultivation may be carried out in liquid broth or on agar plates

Rehydrate and grow freeze dried cells in liquid medium 514 first. Subsequent subculturing may be carried out in medium 514 or tryptone soya broth (medium 545) or on agar media.

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1303 or 514. Subsequent subculturing may be carried out in liquid medium or on agar plates

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 514. Subsequent subculturing may be carried out in liquid medium or on agar plates

Supplemented with 1 g/l pancreatic digest of casein, 1 g/l soya peptone plus 1 g/l malt extract

Supplemented with glucose (1g/L) and vitamin solution of medium 141 (10ml/L); microaerophilic

Takes about 4 weeks to grow

use carbon sources diluted 1:10

Weak growth

With 1 % Na acetate

With 1 % sodium pyruvate

With 1 g/l yeast extract

With 10 mg/L MnSO₄

With 10% NaCl

add with 3 g/l yeast extract, 10 g/l malt extract , 4 g/l Glucose

mix 700 ml marine broth with 300 ml R2A broth, pH 6.5

PH 10

prepare M.514 anaerobically, i.e., boil then bubble with N₂, dispense and autoclave under N₂ atmosphere