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DSMZ

Strain modifications for Medium 898a

4 distinct modifications were found.

Omit D-glucose and increase amount of yeast extract to 2.00 g/l.

Modified strains

Natronincola peptidivorans DSM 18979

Annotated modifications

D-Glucose
Yeast extract
2 g Yeast extract

Omit D-glucose, increase amount of yeast extract to 2.00 g/l and add 2.50 g/l Na2S2O3 x 5 H2O from an anoxic sterile stock solution after autoclaving. Prior to inoculation add 10.00 ml/l of a sterile anoxic 1 M Na-crotonate solution (see medium 870).

Modified strains

Alkaliphilus pronyensis DSM 100017

Annotated modifications

D-Glucose
Yeast extract
2 g Yeast extract
+ 10 ml Na-crotonate solution (1 M)
2.5 g Na2S2O3 x 5 H2O

Replace D-glucose with 5.00 g/l maltose, increase amount of yeast extract to 1.00 g/l and add 2.50 g/l Na2S2O3 x 5 H2O from an anoxic sterile stock solution after autoclaving.

Modified strains

Alkaliphilus serpentinus DSM 100337

Annotated modifications

2.5 g Na2S2O3 x 5 H2O
Yeast extract
D-Glucose
1 g Yeast extract
5 g Maltose

Replace glucose with 2.50 g/l D-mannitol as substrate and adjust pH of complete medium to 9.5 - 9.7.

Modified strains

Anaerobacillus alkalilacustris DSM 18345

Annotated modifications

9.5 - 9.7
D-Glucose
2.5 g D-Mannitol
Modifications
Omit D-glucose and increase amount of yeast extract to 2.00 g/l. Omit D-glucose, increase amount of yeast extract to 2.00 g/l and add 2.50 g/l Na2S2O3 x 5 H2O from an anoxic sterile stock solution after autoclaving. Prior to inoculation add 10.00 ml/l of a sterile anoxic 1 M Na-crotonate solution (see medium 870). Replace D-glucose with 5.00 g/l maltose, increase amount of yeast extract to 1.00 g/l and add 2.50 g/l Na2S2O3 x 5 H2O from an anoxic sterile stock solution after autoclaving. Replace glucose with 2.50 g/l D-mannitol as substrate and adjust pH of complete medium to 9.5 - 9.7.