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DSMZ

Strain modifications for Medium 945

2 distinct modifications were found.

5.00 g/l maltose can be used as substrate instead of sulfur. Maltose is added to autoclaved medium from an anoxic stock solution, sterilized by filtration.

Modified strains

Marinitoga piezophila DSM 14283

Annotated modifications

5 g/l Maltose
Sulfur

Adjust pH of complete medium to 5.5. After inoculation pressurize tubes to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.

Modified strains

Marinitoga okinawensis DSM 17373

Annotated modifications

5.5
33% N2, 53% H2, 13% CO2
Modifications
5.00 g/l maltose can be used as substrate instead of sulfur. Maltose is added to autoclaved medium from an anoxic stock solution, sterilized by filtration. Adjust pH of complete medium to 5.5. After inoculation pressurize tubes to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.