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Main sol. 1002

Internal Solution-ID: S10

# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 3.671
2 0.5 g 0.5 9.338
3 20.0 g 19.98 341.875
4 0.2 g 0.2 0.983
5 0.1 g 0.1 0.9
6 5.0 g 4.995 47.128
7 5.0 g 4.995 59.46
8 1.0 g 0.999 12.178
9 0.1 g 0.1 -
10 0.5 g 0.5 2.013
11 0.1 g 0.1 0.416
12 Trace element solution SL-6 1.0 ml - -
13 20.0 μg 2.0e-5 1.3e-5
14 1000.0 ml 997.21 55353.5
Instructions:
  1. Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5.
1002 ECTOTHIORHODOSYNUS MEDIUM