Main sol. 493
Internal Solution-ID: S1004
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.33 | g | 0.33 | 4.427 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.75 | 13.527 | |
| MgSO4 x 7 H2O | 3.45 | g | 3.45 | 13.997 | |
| NH4Cl | 1.25 | g | 1.25 | 23.369 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
| KH2PO4 | 0.14 | g | 0.14 | 1.029 | |
| NaCl | 0.50 | g | 0.5 | 8.556 | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | 0.015 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except yeast extract and adjust pH to 3.5 with sulfuric acid. After autoclaving add yeast extract from a sterile stock solution. | |||||
Media with this solution
| 493 | THIOMONAS (HÖ5) MEDIUM |