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Main sol. 63c

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Internal Solution-ID: S102

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 980 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary.
63c DESULFOVIBRIO MEDIUM (CHOLINE)