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Main sol. 501

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Internal Solution-ID: S1028

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
Allen's trace element solution 10.00 ml - -
Yeast extract
(BD Bacto) 		1.00 g 1 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
D-Glucose 2.00 g 2 11.101
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except glucose and sulfide), adjust pH at room temperature to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 7.0, if necessary.
501 FERVIDOBACTERIUM ISLANDICUM MEDIUM