MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 502

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1029

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.20 g 1.198 20.493
MgCl2 x 6 H2O 0.40 g 0.399 1.964
KCl 0.30 g 0.299 4.016
CaCl2 x 2 H2O 0.15 g 0.15 1.018
NH4Cl 0.27 g 0.269 5.038
KH2PO4 0.21 g 0.21 1.54
Na2SO4 0.10 g 0.1 0.703
Trace element solution SL-10 1.00 ml - -
Inulin 5.00 g 4.99 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Yeast extract 0.03 g 0.03 -
Casamino acids
(BD Bacto) 		0.03 g 0.03 -
Na2CO3 1.50 g 1.497 14.124
Na2HPO4 2.66 g 2.655 18.7
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.15 g 0.15 0.623
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except yeast extract, Casamino acids, vitamins, carbonate, hydrogenphosphate and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add yeast extract, Casamino acids, hydrogenphosphate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas atmosphere and strerilized by filtration. Adjust pH of complete medium to 7.0, if necessay.
502 PSEUDOCLOSTRIDIUM MEDIUM