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Main sol. 503c

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Internal Solution-ID: S1062

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 922 ml - -
Solution B 50 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
1 Sparge solution A with 100% CO2 gas for 30 – 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and F are autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 6.8, if necessary.
503c AMMONIFEX THIOPHILUS MEDIUM