Main sol. 507
Internal Solution-ID: S1112
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.33 | g | 0.323 | 4.331 | |
MgCl2 x 6 H2O | 0.52 | g | 0.509 | 2.503 | |
CaCl2 x 2 H2O | 0.29 | g | 0.284 | 1.93 | |
NH4Cl | 0.33 | g | 0.323 | 6.037 | |
KH2PO4 | 0.33 | g | 0.323 | 2.373 | |
Trace element solution SL-11 | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
NaHCO3 | 1.00 | g | 0.978 | 11.648 | |
Yeast extract | 0.05 | g | 0.049 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Neutralized sulfide solution 3% (w/v) | 20.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except bicarbonate, yeast extract, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Neutralize the sodium sulfide with sterile H2SO4 (see medium 29). After completing the medium adjust pH to 6.8-7.0. | |||||
2 Inoculated vessels are pressurized with sterile carbon monoxide gas to 2 bar overpressure. |
507 | CARBOXYDOTHERMUS MEDIUM |