Loading ...
Back to all solutions

Main sol. 512

Internal Solution-ID: S1132

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.5 g 0.5 2.871
Na-acetate 1.0 g 1 12.19
NH4Cl 0.1 g 0.1 1.87
Yeast extract 0.1 g 0.1 -
Fe(III) citrate 20.0 ┬ÁM 0.005 0.02
Na-thioglycolate 0.5 g 0.5 4.382
Distilled water 1000.0 ml - -
1 Adjust pH to 6.8. Prepare medium 2 - 3 daysbefore use. Dispense 5 ml of medium in sterile screw capped culture tubes. Inoculate with 10% (v/v) culture. Incubate tubes upright; do not shake.
512 MAGNETOSPIRILLUM II MEDIUM