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Trace element solution (Vishniac and Santer, 1957)

Internal Solution-ID: S114

This solution is used in 5 mediaand was first defined in medium 69.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.
68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM
69 STARKEYA NOVELLA MEDIUM
137 MINERAL LACTATE MEDIUM (low carbon content)
142 THIOMICROSPIRA PELOPHILA MEDIUM
166 HYPHOMICROBIUM STRAIN X MEDIUM