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Main sol. 70

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Internal Solution-ID: S115

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.4 g 0.4 2.939
MgSO4 x 7 H2O 0.4 g 0.4 1.623
(NH4)2SO4 0.4 g 0.4 3.027
FeSO4 x 7 H2O 33.3 g 33.3 119.778
H2SO4
(0.1 N) 		1000.0 ml - -
1 Adjust pH to 1.8 with 2N sulfuric acid. Sterilize by autoclaving for 15 min at 121°C.
70 ACIDITHIOBACILLUS FERROOXIDANS MEDIUM