Main sol. 519
Internal Solution-ID: S1154
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.34 | g | 0.336 | 4.507 | |
MgCl2 x 6 H2O | 4.00 | g | 3.953 | 19.442 | |
MgSO4 x 7 H2O | 3.45 | g | 3.409 | 13.831 | |
Na2SO4 | 2.70 | g | 2.668 | 18.783 | |
NH4Cl | 0.25 | g | 0.247 | 4.618 | |
CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.941 | |
K2HPO4 | 0.14 | g | 0.138 | 0.794 | |
NaCl | 18.00 | g | 17.787 | 304.356 | |
Yeast extract
(OXOID) |
0.50 | g | 0.494 | - | |
Na-acetate | 1.00 | g | 0.988 | 12.046 | |
Fe(NH4)2(SO4)2 x 7 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | 0.005 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
NaHCO3 | 1.00 | g | 0.988 | 11.763 | |
Na2S x 9 H2O | 0.50 | g | 0.494 | 2.057 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except bicarbonate and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate, adjust pH to 6.5 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic serum vials (e.g., 20 ml medium per 100 ml bottle), pressurize bottles up to 2 bar overpressure, then autoclave. Before use, release excess pressure and reduce the medium with sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||
2 After inoculation, add 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture. |
519 | ARCHAEOGLOBUS PROFUNDUS MEDIUM |