MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 71

Download this solution as

CSV JSON PDF

Internal Solution-ID: S116

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 3.00 g 3 22.045
MgSO4 x 7 H2O 0.50 g 0.5 2.029
(NH4)2SO4 3.00 g 3 22.703
CaCl2 x 2 H2O 0.25 g 0.25 1.701
Na2S2O3 x 5 H2O 5.00 g 5 20.146
Distilled water 1000.00 ml - -
1 Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving.
2 Note: Incubate statically without shaking.
71 ACIDITHIOBACILLUS THIOOXIDANS MEDIUM