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Trace elements solution VR

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Internal Solution-ID: S1163

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		5.00 g 5 26.159
MgSO4 x 7 H2O 24.00 g 24 97.373
CaCl2 x 2 H2O 0.50 g 0.5 3.401
FeSO4 x 7 H2O 0.50 g 0.5 1.798
NiCl2 x 6 H2O 0.25 g 0.25 1.929
ZnSO4 x 7 H2O 0.44 g 0.44 1.53
MnSO4 x H2O 0.25 g 0.25 1.479
CoCl2 x 6 H2O 0.25 g 0.25 1.925
VOSO4 x 5 H2O 0.25 g 0.25 0.988
Na2MoO4 x 2 H2O 0.25 g 0.25 1.033
CuSO4 x 5 H2O 0.12 g 0.12 0.481
Distilled water 1000.00 ml - -
1 Dissolve mineral salts in 700 ml water acidified with 2 ml of conc. HCl. Separately dissolve 5.00 g of NTA in 300 ml water add 10 N NaOH until all the NTA has dissolved and the pH is 7.0. While stirring, slowly add the NTA solution to the salt solution. Stir until the solution is almost clear. Store cool and dark.
526 CLOSTRIDIUM VIRIDE MEDIUM