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Main sol. 530

Internal Solution-ID: S1175

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 5.0 g 5 -
Meat extract 3.0 g 3 -
Na-thioglycolate 0.5 g 0.5 4.382
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 Adjust pH to 5.5.
2 Sterilize Na-Thioglycolate separately by filtration.
3 Optimal pO2 for growth on solid media is 0.03 - 0.08 atm.
530 THIOGLYCOLATE MEDIUM