Main sol. 539
Internal Solution-ID: S1193
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.2 | 1.468 | |
NH4Cl | 0.30 | g | 0.3 | 5.603 | |
NaCl | 1.00 | g | 0.999 | 17.095 | |
MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.966 | |
KCl | 0.50 | g | 0.5 | 6.7 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.019 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Yeast extract | 0.50 | g | 0.5 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.00 | g | 0.999 | 9.426 | |
Cellobiose | 5.00 | g | 4.995 | 14.593 | |
Cellulose
, MN 301
optional |
5.00 | g | 4.995 | - | |
Na2S x 9 H2O | 0.40 | g | 0.4 | 1.664 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, cellobiose and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0, if necessary. | |||||
2 Note: Some strains can be adapted to cellulose as substrate using 5.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL). |
539 | RUMINICLOSTRIDIUM CELLOBIOPARUM MEDIUM |