Main sol. 76
Internal Solution-ID: S121
Equipment needed: Hungate tubes
Filter
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KOH | 0.67 | g | 0.67 | 11.942 | |
| K2HPO4 | 0.91 | g | 0.91 | 5.225 | |
| Uric acid | 2.00 | g | 2 | 11.897 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 15.00 | mg | 0.015 | 0.102 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
6.00 | ml | 0.006 | 0.022 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
| Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other compounds, except carbonate and thioglycolate. Dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Then add carbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and thioglycolate (stock solution, autoclaved separately under 100% N2 gas). Adjust pH of complete medium to 7.0 - 7.5, if necessary. | |||||
Media with this solution
| 76 | GOTTSCHALKIA MEDIUM |