Main sol. 558a
Internal Solution-ID: S1220
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.50 | g | 1.484 | 10.903 | |
| Na2HPO4 x 12 H2O | 4.20 | g | 4.154 | 29.264 | |
| NH4Cl | 0.50 | g | 0.495 | 9.246 | |
| MgCl2 x 6 H2O | 0.36 | g | 0.356 | 1.751 | |
| Na2SO4 | 1.00 | g | 0.989 | 6.964 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| D-Glucose | 10.00 | g | 9.891 | 54.902 | |
| Yeast extract | 2.00 | g | 1.978 | - | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | 2.816 | |
| Na2S x 9 H2O | 0.50 | g | 0.495 | 2.059 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except glucose, yeast extract, vitamin solution and reducing agents), adjust pH to 5 - 6 using HCl and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose, yeast extract, vitamin solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 5.0 - 6.0, if necessary. | |||||
Media with this solution
| 558a | CLOSTRIDIUM (RB-9) MEDIUM |