Main sol. 579
Internal Solution-ID: S1244
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Ferric citrate monohydrate | 13.7 | g | 13.551 | 51.532 | |
| NH4Cl | 1.5 | g | 1.484 | 27.738 | |
| NaH2PO4 | 0.6 | g | 0.593 | 4.947 | |
| KCl | 0.1 | g | 0.099 | 1.327 | |
| Na-acetate | 2.5 | g | 2.473 | 30.144 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| NaHCO3 | 2.5 | g | 2.473 | 29.436 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 First dissolve ferric citrate in water by heating and adjust to pH 6.0, then add other medium ingredients except bicarbonate and vitamins. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then add and dissolve bicarbonate and adjust pH to 6.8. Thereafter, dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins to the sterile medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 6.7 - 7.0, if necessary. | |||||
| 2 Note: Use 5 - 10% (v/v) inoculum. | |||||
Media with this solution
| 579 | GEOBACTER MEDIUM |