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Main sol. 582

Internal Solution-ID: S1247

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 121.500 g 121.5 2079.06
MgCl2 10.500 g 10.5 110.283
MgSO4 14.400 g 14.4 119.631
CaCl2 0.540 g 0.54 4.866
KCl 3.000 g 3 40.241
NaHCO3 0.090 g 0.09 1.071
NaBr 0.039 g 0.039 0.379
Proteose peptone no. 3
(Difco)
5.000 g 5 -
Yeast extract
(Difco)
10.000 g 10 -
Glucose 1.000 g 1 5.551
Distilled water 1000.000 ml - -
1 Final pH 7.5
2 NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 20 g/l for solid media.
582 15% MH MEDIUM