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Hutner's salts

Internal Solution-ID: S1268

This solution is used in 25 mediaand was first defined in medium 590.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
590 DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM
600b M13 (3x) PIRELLULA MEDIUM
600a M13a (PIRELLULA) MEDIUM
600 M14 (PIRELLULA MARINA) MEDIUM
602 HALOMONAS SUBGLACIESCOLA
607 M13 VERRUCOMICROBIUM MEDIUM
628 MMB MEDIUM
629 STALEY'S MAINTENANCE MEDIUM
768 M17 MEDIUM FOR FILOMICROBIUM FUSIFORME
773 PARACOCCUS KOCURII MEDIUM
1327 LARKINELLA ARBORICOLA MEDIUM
1367 TELMATOCOLA MEDIUM
1419 HYPHOMICROBIUM MEDIUM
1420 HALF STRENGHS HYPHOMICROBIUM MEDIUM
1436 PIRELLULA MEDIUM (M31PY)
1437 ALKALIPHILES PIRELLULA MEDIUM (M1PY)
1438 PIRELLULA MEDIUM (M31 PY)
1439 ALKALIPHILES PIRELLULA MEDIUM (M1aPY)
1440 PIRELLULA MEDIUM (M31)
1442 PIRELLULA MEDIUM (M33a)
1443 PIRELLULA MEDIUM (M30 PY)
1444 PIRELLULA MEDIUM (M30a)
1544 . PIRELLULA MEDIUM (M30)
1601 MODIFIED M13 VERRUCOMICROBIUM MEDIUM
1728 MMB MEDIUM (ATCC 2254)