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Hutner's salts
Internal Solution-ID: S1268
This solution is used in 25 media and was first defined in medium 590.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
Media with this solution
| 590 | DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM |
| 600b | M13 (3x) PIRELLULA MEDIUM |
| 600a | M13a (PIRELLULA) MEDIUM |
| 600 | M14 (PIRELLULA MARINA) MEDIUM |
| 602 | HALOMONAS SUBGLACIESCOLA |
| 607 | M13 VERRUCOMICROBIUM MEDIUM |
| 628 | MMB MEDIUM |
| 629 | STALEY'S MAINTENANCE MEDIUM |
| 768 | M17 MEDIUM FOR FILOMICROBIUM FUSIFORME |
| 773 | PARACOCCUS KOCURII MEDIUM |
| 1327 | LARKINELLA ARBORICOLA MEDIUM |
| 1367 | TELMATOCOLA MEDIUM |
| 1419 | HYPHOMICROBIUM MEDIUM |
| 1420 | HALF STRENGHS HYPHOMICROBIUM MEDIUM |
| 1436 | PIRELLULA MEDIUM (M31PY) |
| 1437 | ALKALIPHILES PIRELLULA MEDIUM (M1PY) |
| 1438 | PIRELLULA MEDIUM (M31 PY) |
| 1439 | ALKALIPHILES PIRELLULA MEDIUM (M1aPY) |
| 1440 | PIRELLULA MEDIUM (M31) |
| 1442 | PIRELLULA MEDIUM (M33a) |
| 1443 | PIRELLULA MEDIUM (M30 PY) |
| 1444 | PIRELLULA MEDIUM (M30a) |
| 1544 | . PIRELLULA MEDIUM (M30) |
| 1601 | MODIFIED M13 VERRUCOMICROBIUM MEDIUM |
| 1728 | MMB MEDIUM (ATCC 2254) |