MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 591

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1270

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 0.33 g 0.33 2.974
MgCl2 0.33 g 0.33 3.466
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
NaCl , adjust if required in range 100-150 125.00 g 125 2138.95
NaHCO3 1.50 g 1.5 17.856
Na2S x 9 H2O 0.50 g 0.5 2.082
Glucose 5.00 g 5 27.753
Peptone
(Bacto Proteose Peptone No.3) 		5.00 g 5 -
Trace element solution SL-10
(SL 10) 		1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Resazurin 2.00 mg 0.002 0.009
Distilled water 990.00 ml - -
1 Prepare the medium without NaHCO3, Na2S, glucose and vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Glucose (filter-sterilized and stored under nitrogen), vitamins (filter sterilized and stored under nitrogen), and sodium sulphide (autoclaved under nitrogen) are added from sterile stock solutions. The pH should be pH 7.5.
591 HALOBACTEROIDES/HALOINCOLA MEDIUM