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Main sol. 603

Internal Solution-ID: S1295

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.250 g 0.248 1.873
Glucose 0.250 g 0.248 1.374
Vitamin solution 10.000 ml - -
Hutner's salts 20.000 ml - -
Na2HPO4 0.071 g 0.07 0.495
Distilled water 980.000 ml - -
Agar , for solid medium 15.000 g/l 15 44.599
1 pH 7
2 Make up the medium without the vitamin solution and autoclave it. The filter-sterilized vitamin solution is added to the cooled medium. The medium may be solidified by adding 15 g/l agar.
603 ANCALOMICROBIUM MEDIUM