Main sol. 611
Internal Solution-ID: S1311
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Synthetic seawater mix | 125.00 | ml | - | - | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O
(0.01% w/v) |
2.00 | ml | 2.0e-4 | - | |
NH4Cl | 0.25 | g | 0.25 | 4.674 | |
KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
KNO3 | 1.00 | g | 1 | 9.891 | |
Yeast extract | 0.50 | g | 0.5 | - | |
NaHCO3 | 2.00 | g | 2 | 23.808 | |
Distilled water | 875.00 | ml | - | - | |
1 Dissolve ingredients (except nitrate, yeast extract and bicarbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.0 and dispense under 100% N2 gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle). Replace gas phase with 80% H2 and 20% CO2 gas mixture and autoclave. Prior to inoculation add nitrate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2. |
611 | PYROBACULUM (BS) MEDIUM |