Main sol. 613
Internal Solution-ID: S1319
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.50 | g | 0.495 | 9.246 | |
| K2HPO4 | 1.60 | g | 1.583 | 9.086 | |
| Na2HPO4 x H2O | 1.00 | g | 0.989 | 6.968 | |
| NaCl | 7.00 | g | 6.924 | 118.478 | |
| MgCl2 x 6 H2O | 1.40 | g | 1.385 | 6.811 | |
| MgSO4 x 7 H2O | 1.96 | g | 1.939 | 7.866 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.978 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.978 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| D-Glucose | 3.00 | g | 2.967 | 16.471 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.059 | 0.404 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.297 | 1.689 | |
| Na2S x 9 H2O | 0.30 | g | 0.297 | 1.235 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except glucose, calcium chloride, vitamins and reducing agents), adjust pH to 6.5 - 6.8 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add glucose, calcium chloride, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. | |||||
Media with this solution
| 613 | THERMOTOGA TF(C) MEDIUM |