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Main sol. 629

Internal Solution-ID: S1343

Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5.0 g 5 -
Yeast extract 0.5 g 0.5 -
Hutner's salts 20.0 ml - -
Vitamin solution
(filter-sterilized)
10.0 ml - -
Distilled water 970.0 ml - -
Agar , for solid medium 15.0 g/l 15 44.599
1 Dissolve peptone and yeast extract in the distilled water, adjust the pH to 7.2 and sterilize. After the medium has cooled add the Hutner's salts and Vitamin solution.
2 For solid medium use 15.0 g agar.
629 STALEY'S MAINTENANCE MEDIUM