Main sol. 629
Internal Solution-ID: S1343
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 5.0 | g | 5 | - | |
Yeast extract | 0.5 | g | 0.5 | - | |
Hutner's salts | 20.0 | ml | - | - | |
Vitamin solution
(filter-sterilized) |
10.0 | ml | - | - | |
Distilled water | 970.0 | ml | - | - | |
Agar , for solid medium | 15.0 | g/l | 15 | 44.599 | |
1 Dissolve peptone and yeast extract in the distilled water, adjust the pH to 7.2 and sterilize. After the medium has cooled add the Hutner's salts and Vitamin solution. | |||||
2 For solid medium use 15.0 g agar. |
629 | STALEY'S MAINTENANCE MEDIUM |