Main sol. 630
Internal Solution-ID: S1345
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 2.5 | g | 2.5 | - | |
Tryptone | 2.5 | g | 2.5 | 35.172 | |
Agar | 28.0 | g | 28 | 83.252 | |
Nitrilotriacetic acid | 100.0 | mg | 0.1 | 0.523 | |
CaSO4 x 2 H2O | 40.0 | mg | 0.04 | 0.232 | |
MgCl2 x 6 H2O | 200.0 | mg | 0.2 | 0.984 | |
Fe(III) citrate
(0.01 M) |
0.5 | ml | 0.001 | 0.005 | |
Trace element solution | 0.5 | ml | - | - | |
Phosphate buffer | 100.0 | ml | - | - | |
Distilled water | 900.0 | ml | - | - | |
1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium. |
630 | MODIFIED THERMUS 162 MEDIUM |