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Main sol. 630

Internal Solution-ID: S1345

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 2.5 g 2.5 -
Tryptone 2.5 g 2.5 35.172
Agar 28.0 g 28 83.252
Nitrilotriacetic acid 100.0 mg 0.1 0.523
CaSO4 x 2 H2O 40.0 mg 0.04 0.232
MgCl2 x 6 H2O 200.0 mg 0.2 0.984
Fe(III) citrate
(0.01 M)
0.5 ml 0.001 0.005
Trace element solution 0.5 ml - -
Phosphate buffer 100.0 ml - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium.
630 MODIFIED THERMUS 162 MEDIUM