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Trace element solution

Internal Solution-ID: S1347

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.8 g 12.8 66.967
FeCl2 x 4 H2O 1.0 g 1 5.03
MnCl2 x 4 H2O 0.5 g 0.5 2.526
CoCl2 x 4 H2O 0.3 g 0.3 2.311
CuCl2 x 2 H2O 50.0 mg 0.05 0.293
Na2MoO4 x 2 H2O 50.0 mg 0.05 0.207
H3BO3 20.0 mg 0.02 0.323
NiCl2 x 6 H2O 20.0 mg 0.02 0.154
Distilled water 1000.0 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
630 MODIFIED THERMUS 162 MEDIUM