Trace element solution
Internal Solution-ID: S1347
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.8 | g | 12.8 | 66.967 | |
FeCl2 x 4 H2O | 1.0 | g | 1 | 5.03 | |
MnCl2 x 4 H2O | 0.5 | g | 0.5 | 2.526 | |
CoCl2 x 4 H2O | 0.3 | g | 0.3 | 2.311 | |
CuCl2 x 2 H2O | 50.0 | mg | 0.05 | 0.293 | |
Na2MoO4 x 2 H2O | 50.0 | mg | 0.05 | 0.207 | |
H3BO3 | 20.0 | mg | 0.02 | 0.323 | |
NiCl2 x 6 H2O | 20.0 | mg | 0.02 | 0.154 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. |
630 | MODIFIED THERMUS 162 MEDIUM |