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Main sol. 634

Internal Solution-ID: S1352

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
dehydrated RCM medium
(dissolved in distilled water)
1000.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.