Main sol. 634
Internal Solution-ID: S1352
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
dehydrated RCM medium
(dissolved in distilled water) |
1000.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| 1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Media with this solution
| 634 | RCM MEDIUM |