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Main sol. 641

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Internal Solution-ID: S1366

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.997 18.639
Na2SO4 2.0 g 1.994 14.038
Na2S2O3 x 5 H2O 1.0 g 0.997 4.017
MgSO4 x 7 H2O 1.0 g 0.997 4.045
CaCl2 x 2 H2O 0.1 g 0.1 0.678
KH2PO4 0.5 g 0.499 3.663
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 0.997 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 1.0 g 0.997 9.407
Na-DL-lactate 2.5 g 2.493 22.243
Wolin's vitamin solution (10x) 1.0 ml - -
Na2S x 9 H2O 0.1 g 0.1 0.415
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, vitamins, lactate and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
641 DESULFOVIBRIO (MV) MEDIUM