Main sol. 664a
Internal Solution-ID: S1397
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| NaCl | 10.0 | g | 10 | 171.116 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Yeast extract | 0.5 | g | 0.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.5 | g | 1.5 | 14.153 | |
| Na2S2O3 x 5 H2O | 5.0 | g | 5 | 20.146 | |
| D-Glucose | 4.0 | g | 4 | 22.203 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, thiosulfate, glucose and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The pH of the complete medium should be adjusted to 7.0, if necessary. | |||||
Media with this solution
| 664a | PSEUDOTHERMOTOGA LETTINGAE MEDIUM |