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Solution A

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Internal Solution-ID: S1399

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 4.286 32.433
KCl 0.10 g 0.143 1.916
K2HPO4 0.50 g 0.714 4.101
MgSO4 x 7 H2O 0.50 g 0.714 2.898
Ca(NO3)2 0.01 g 0.014 0.087
Distilled water 700.00 ml - -
1 Adjust pH to 2.0 - 2.2 with sulfuric acid.
665 SULFOBACILLUS MEDIUM