1
Dissolve ingredients (except bicarbonate, succinate, yeast extract, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add succinate, yeast extract, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.7 - 6.8, if necessary.
2
Note: Supplementing the medium with 1.50 g/l agar stimulates growth of strains after resuscitation from ampoules.
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