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Main sol. 667

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Internal Solution-ID: S1404

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 973 ml - -
Solution B 30 ml - -
Solution C 1 ml - -
Solution D 5 ml - -
Solution E 1 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution D is autoclaved under 100% N2 gas. Solution E is prepared under 80% N2 and 20% CO2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.0 - 7.2.
667 DESULFALLAS THERMOSAPOVORANS MEDIUM