MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 81a

Download this solution as

CSV JSON PDF

Internal Solution-ID: S142

Equipment needed: Gassing station Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.30 g 2.275 13.061
Na2HPO4 x 2 H2O 2.90 g 2.868 20.206
NH4Cl 1.00 g 0.989 18.492
CaCl2 x 2 H2O 0.01 g 0.01 0.067
MgSO4 x 7 H2O 0.50 g 0.495 2.007
NaVO3 x H2O
(0.1% w/v) 		5.00 ml 0.005 0.035
Trace element solution SL-6 5.00 ml - -
NaHCO3 0.50 g 0.495 5.887
Ferric ammonium citrate 0.05 g 0.049 -
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (ecxept bicarbonate, ferric ammonium citrate and vitamins), then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Adjust pH to 4.5 and dispense solution under air atmosphere into serum vials suitable for anaerobic cultivation to 50% of volume. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add ferric ammonium citrate and vitamins from anoxic stock solutions sterilized by filtration and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 5.0, if necessary.
2 After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
3 Note: Use at least 10% (v/v) as inoculum.
81a THIOMONAS ISLANDICA MEDIUM